Reprint of: A chemical screen identifies trifluoperazine as an inhibitor of glioblastoma growth

Biochem Biophys Res Commun. 2018 May 5;499(2):136-142. doi: 10.1016/j.bbrc.2018.03.001. Epub 2018 Mar 23.


Glioblastoma (GBM) is regarded as the most common malignant brain tumor but treatment options are limited. Thus, there is an unmet clinical need for compounds and corresponding targets that could inhibit GBM growth. We screened a library of 80 dopaminergic ligands with the aim of identifying compounds capable of inhibiting GBM cell line proliferation and survival. Out of 45 active compounds, 8 were further validated. We found that the dopamine receptor D2 antagonist trifluoperazine 2HCl inhibits growth and proliferation of GBM cells in a dose dependent manner. Trifluoperazine's inhibition of GBM cells is cell line dependent and correlates with variations in dopamine receptor expression profile. We conclude that components of the dopamine receptor signaling pathways are potential targets for pharmacological interventions of GBM growth.

Keywords: 6,7-ADTN HBr (PubChem CID: 11230706); Bromocriptine Mesylate (PubChem CID: 31100); Cancer; Cell lines; Chlorpromazine HCl (PubChem CID: 6240); Clozapine (PubChem CID: 2818); Dopamine; Glioblastoma; R(−)-Apomorphine (PubChem CID: 6005); S(−)-Raclopride l-Tartrate (PubChem CID: 11663304); SKF-83566 HCl (PubChem CID: 1243); Screening; Trifluoperazine; Trifluoperazine 2HCl (PubChem CID: 66064).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Count
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Proliferation / genetics
  • Cell Survival / drug effects
  • Cell Survival / genetics
  • Dopamine / metabolism
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical*
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / drug effects
  • Glioblastoma / genetics
  • Glioblastoma / pathology*
  • Humans
  • Ligands
  • Receptors, Dopamine / genetics
  • Receptors, Dopamine / metabolism
  • Signal Transduction / drug effects
  • Trifluoperazine / chemistry
  • Trifluoperazine / pharmacology*


  • Ligands
  • Receptors, Dopamine
  • Trifluoperazine
  • Dopamine