The E3/19K protein of adenovirus type 2 binds to the domains of histocompatibility antigens required for CTL recognition

EMBO J. 1987 Jul;6(7):2019-26.

Abstract

The E3/19K protein of human adenovirus type 2 binds to HLA class I antigens and blocks their terminal glycosylation and cell surface expression. The nature of this interaction is non-covalent and involves neither disulfide bridges between the two molecules nor their carbohydrates. The murine H-2 Kd antigen associates with the E3/19K protein in a similar fashion to human HLA antigens whereas the allelic product H-2 Kk does not. Hybrid genes between the Kd and Kk alleles were constructed and their products were expressed in embryonic kidney cells together with the E3/19K protein. This allowed us to identify the alpha 1 and alpha 2 domains as the essential structures of the histocompatibility antigens for binding the viral protein. Interestingly, these domains are also crucial for T cell recognition. The implications for the evolution of adenoviruses and their ability to cause persistent infections are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Early Proteins
  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / immunology*
  • Animals
  • Antigens, Viral, Tumor / immunology*
  • Biological Evolution
  • Cell Line
  • Cytotoxicity, Immunologic*
  • H-2 Antigens / genetics
  • H-2 Antigens / immunology
  • HLA Antigens / immunology*
  • Oncogene Proteins, Viral / immunology*

Substances

  • Adenovirus Early Proteins
  • Antigens, Viral, Tumor
  • H-2 Antigens
  • HLA Antigens
  • Oncogene Proteins, Viral