Stool DNA Integrity Method for Colorectal Cancer Detection

Methods Mol Biol. 2018:1765:193-202. doi: 10.1007/978-1-4939-7765-9_12.

Abstract

Fluorescence long DNA (FL-DNA) is a non-invasive and simple-to-perform stool DNA test. This assay consists of a qualitative and quantitative real-time PCR (RT PCR) analysis. FL-DNA has great potential in colorectal cancer (CRC) lesions detection used alone or in combination with the standard CRC screening tool: immunochemical fecal occult blood test (iFOBT).

Keywords: CRC; Colonocytes; FL-DNA; Screening; Stool DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Colorectal Neoplasms / diagnosis*
  • DNA / analysis*
  • Early Detection of Cancer / instrumentation
  • Early Detection of Cancer / methods*
  • Feces / chemistry*
  • Humans
  • Occult Blood
  • Real-Time Polymerase Chain Reaction / instrumentation
  • Real-Time Polymerase Chain Reaction / methods*
  • Time Factors

Substances

  • DNA