Phytochelatins, the heavy metal binding peptides of plants: characterization and sequence determination

Experientia Suppl. 1987;52:317-22. doi: 10.1007/978-3-0348-6784-9_28.

Abstract

The isolation of a homologous series of five novel heavy metal complexing peptides from plant cell suspension cultures is reported. Their structures were established as (gamma-Glu-Cys)n Gly, n = 3-7. These peptides appear upon induction with heavy metals and represent the principal metal binding activity in induced cells. The name phytochelatin (PC) is proposed for this new class of natural products in higher plants. The hitherto postulated metallothioneins do not exist in plants but their function of chelating heavy metals via metal-thiolate coordination is fulfilled by the simpler phytochelatins. They are not primary gene products because of their repetition of gamma-glutamyl linkages.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Cadmium / metabolism
  • Cadmium / pharmacology
  • Chromatography, High Pressure Liquid
  • Circular Dichroism
  • Electrophoresis, Polyacrylamide Gel
  • Metals / metabolism*
  • Metals / pharmacology
  • Molecular Sequence Data
  • Oligopeptides / biosynthesis
  • Oligopeptides / isolation & purification*
  • Plant Proteins / biosynthesis
  • Plant Proteins / isolation & purification*
  • Plants / analysis*
  • Plants / drug effects

Substances

  • Amino Acids
  • Metals
  • Oligopeptides
  • Plant Proteins
  • Cadmium