Lymphoblast-derived integration-free iPSC line AD-TREM2-1 from a 67year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant

Stem Cell Res. 2018 May;29:60-63. doi: 10.1016/j.scr.2018.03.011. Epub 2018 Mar 20.

Abstract

Human lymphoblast cells from a male diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. AD-TREM2-1 was defined as pluripotent based on (i) expression of pluripotency-associated markers (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptome of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0.947.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Alzheimer Disease / metabolism
  • Cell Differentiation
  • Cell Line
  • Humans
  • Induced Pluripotent Stem Cells / metabolism*
  • Male
  • Membrane Glycoproteins / metabolism*
  • Receptors, Immunologic / metabolism*

Substances

  • Membrane Glycoproteins
  • Receptors, Immunologic
  • TREM2 protein, human