Transcription of Neurospora crassa 5 S rRNA genes requires a TATA box and three internal elements

J Mol Biol. 1987 Aug 20;196(4):801-11. doi: 10.1016/0022-2836(87)90406-2.

Abstract

The sequences required for transcription of Neurospora crassa 5S rRNA genes have been defined using a comprehensive set of deletion and substitution mutations introduced into two cloned genes. An upstream TATA box (consensus TCATAGA) located at -29 to -24, and three internal regions (D, A and C) localized to +19 to +30, +44 to +57 and +73 to +103, respectively, are absolutely required for transcription in vitro. The TATA box fixes the start point of transcription. The A and C regions correspond to the Xenopus 5S gene internal control region but the D region has no Xenopus homologue. The spacing of the three internal elements is very strict but the position of the TATA box can be varied by up to 16 base-pairs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Genes, Fungal*
  • Molecular Sequence Data
  • Mutation
  • Neurospora / genetics*
  • Neurospora crassa / genetics*
  • Promoter Regions, Genetic
  • RNA, Fungal / genetics*
  • RNA, Ribosomal / genetics*
  • RNA, Ribosomal, 5S / genetics*
  • Transcription, Genetic*

Substances

  • RNA, Fungal
  • RNA, Ribosomal
  • RNA, Ribosomal, 5S