ChIP is a powerful method for analyzing protein-DNA interactions that occur in vivo. A basic protocol involves immunoprecipitation of the cross-linked nucleoprotein complex and isolating and analyzing the DNA that is associated with the protein of interest. The antibody and chromatin amounts indicated here are for ChIP-chip and ChIP-seq and are ∼10-fold higher than for a standard ChIP assay.
© 2018 Cold Spring Harbor Laboratory Press.