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. 2018 Apr;4(4):218-224.
doi: 10.1038/s41477-018-0130-0. Epub 2018 Apr 2.

X-ray Structure of an Asymmetrical Trimeric Ferredoxin-Photosystem I Complex

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X-ray Structure of an Asymmetrical Trimeric Ferredoxin-Photosystem I Complex

Hisako Kubota-Kawai et al. Nat Plants. .

Abstract

Photosystem I (PSI), a large protein complex located in the thylakoid membrane, mediates the final step in light-driven electron transfer to the stromal electron carrier protein ferredoxin (Fd). Here, we report the first structural description of the PSI-Fd complex from Thermosynechococcus elongatus. The trimeric PSI complex binds three Fds in a non-equivalent manner. While each is recognized by a PSI protomer in a similar orientation, the distances between Fds and the PSI redox centres differ. Fd binding thus entails loss of the exact three-fold symmetry of the PSI's soluble subunits, inducing structural perturbations which are transferred to the lumen through PsaF. Affinity chromatography and nuclear magnetic resonance analyses of PSI-Fd complexes support the existence of two different Fd-binding states, with one Fd being more tightly bound than the others. We propose a dynamic structural basis for productive complex formation, which supports fast electron transfer between PSI and Fd.

Comment in

  • Electron handoff.
    Deane C. Deane C. Nat Chem Biol. 2018 Jun;14(6):525. doi: 10.1038/s41589-018-0073-9. Nat Chem Biol. 2018. PMID: 29769730 No abstract available.

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