An antibody to the receptor for insulin-like growth factor I inhibits the growth of MCF-7 cells in tissue culture

Biochem Biophys Res Commun. 1987 Nov 30;149(1):276-81. doi: 10.1016/0006-291x(87)91635-4.

Abstract

Alpha IR-3, a monoclonal antibody to the insulin-like growth factor I receptor which blocks insulin-like growth factor I binding and inhibits its activity, inhibits the binding of 125I-insulin-like growth factor I to MCF-7 cells (an estrogen dependent human breast carcinoma cell line) with an IC-50 of approximately 100 ng/ml. It also inhibits the growth of MCF-7 cells cultured in 5% calf serum with approximately the same IC-50. Inhibition of growth occurs both when cells are cultured in the presence and absence of estrogen and is more pronounced when cells are grown at a low density. These findings demonstrate a requirement for insulin-like growth factor I for optimal growth of MCF-7 cells and suggest that it is an autocrine growth factor in these cells.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / physiology*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Cell Count
  • Cell Division / drug effects
  • Estradiol / pharmacology
  • Humans
  • Insulin-Like Growth Factor I / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Receptor, Insulin / immunology
  • Receptor, Insulin / physiology*
  • Receptors, Somatomedin
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Receptors, Somatomedin
  • Estradiol
  • Insulin-Like Growth Factor I
  • Receptor, Insulin