Selective cytotoxicity of phospholipids and diacylglycerols to rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene

Cancer Res. 1988 Feb 1;48(3):578-83.


The colony-forming ability of rat 3Y1 fibroblasts transformed by adenovirus type 12 (Ad12) was drastically reduced when the cells were cultivated for 18 h in medium augmented with 300 micrograms/ml of liposomes composed of either phosphatidylcholine (PC) or phosphatidylinositol. In contrast, those of untransformed 3Y1 cells and simian virus 40-transformed and polyomavirus-transformed 3Y1 cells were not. The cytotoxicity of PC liposomes was also observed in 3Y1 cells transformed by plasmid DNA containing Ad12-E1A gene but not in those transformed by adenovirus type 2, Rous avian sarcoma virus, or plasmid DNA carrying v-Ha-ras oncogene. The extensive killing of Ad12-transformed and E1A-transformed 3Y1 cells occurred in liposomes of dioleoyl-PC and of dilinoleoyl PC but not those of dipalmitoyl PC, distearoyl-PC, or diarachidonyl PC, suggesting that the acyl groups of phospholipids play an important role in cytotoxicity. Dilinoleoylglycerol, 60 micrograms/ml, was also cytotoxic selectively to Ad12-transformed and E1A-transformed 3Y1 cells, although the toxicity of lysophosphatidylcholine or linoleic acid was not specific to these transformants. These results suggest that cell transformation by Ad12 is characterized by a high sensitivity to exogenously administered phospholipids and diacylglycerol that contain oleoyl or linoleoyl acyl groups and that the sensitivity is attributable to the expression of E1A gene of Ad12.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Early Proteins
  • Adenoviruses, Human
  • Cell Division / drug effects*
  • Cell Survival / drug effects*
  • Cell Transformation, Viral*
  • Diglycerides / toxicity*
  • Fatty Acids, Nonesterified / toxicity
  • Glycerides / toxicity*
  • Liposomes
  • Lysophosphatidylcholines / toxicity
  • Oncogene Proteins, Viral
  • Phosphatidylcholines / toxicity
  • Phospholipids / toxicity*
  • Structure-Activity Relationship


  • Adenovirus Early Proteins
  • Diglycerides
  • Fatty Acids, Nonesterified
  • Glycerides
  • Liposomes
  • Lysophosphatidylcholines
  • Oncogene Proteins, Viral
  • Phosphatidylcholines
  • Phospholipids