Sulforaphane Inhibits the Generation of Amyloid-β Oligomer and Promotes Spatial Learning and Memory in Alzheimer's Disease (PS1V97L) Transgenic Mice

J Alzheimers Dis. 2018;62(4):1803-1813. doi: 10.3233/JAD-171110.

Abstract

Abnormal amyloid-β (Aβ) aggregates are a striking feature of Alzheimer's disease (AD), and Aβ oligomers have been proven to be crucial in the pathology of AD. Any intervention targeting the generation or aggregation of Aβ can be expected to be useful in AD treatment. Oxidative stress and inflammation are common pathological changes in AD that are involved in the generation and aggregation of Aβ. In the present study, 6-month-old PS1V97L transgenic (Tg) mice were treated with sulforaphane, an antioxidant, for 4 months, and this treatment significantly inhibited the generation and aggregation of Aβ. Sulforaphane also alleviated several downstream pathological changes that including tau hyperphosphorylation, oxidative stress, and neuroinflammation. Most importantly, the cognition of the sulforaphane-treated PS1V97L Tg mice remained normal compared to that of wild-type mice at 10 months of age, when dementia typically emerges in PS1V97L Tg mice. Pretreating cultured cortical neurons with sulforaphane also protected against neuronal injury caused by Aβ oligomers in vitro. These findings suggest that sulforaphane may be a potential compound that can inhibit Aβ oligomer production in AD.

Keywords: Alzheimer’s disease; Aβ oligomer; inflammation; oxidative stress; sulforaphane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy*
  • Alzheimer Disease / metabolism
  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Antioxidants / pharmacology*
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Female
  • Humans
  • Inflammation / drug therapy
  • Inflammation / metabolism
  • Isothiocyanates / pharmacology*
  • Male
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Nootropic Agents / pharmacology
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology
  • Presenilin-1 / genetics
  • Presenilin-1 / metabolism
  • Primary Cell Culture
  • Rats, Sprague-Dawley
  • Spatial Learning / drug effects*
  • Spatial Learning / physiology
  • Spatial Memory / drug effects*
  • Spatial Memory / physiology
  • Sulfoxides

Substances

  • Amyloid beta-Peptides
  • Antioxidants
  • Isothiocyanates
  • Nootropic Agents
  • PSEN1 protein, human
  • Presenilin-1
  • Sulfoxides
  • sulforaphane