In the human T-cell rearranging gamma (TRG gamma) locus, five joining (J) segments have been identified: J1, J2 and three additional segments JP, JP1 and JP2. We report the sequence of the germline JP1 segment and compare it with the other human and mouse J gamma segments. We also demonstrate that rearrangements to the three additional J gamma segments can be identified by hybridization of the KpnI digests to the J gamma 1 probe pH60. Since rearrangements to J1 or J2 can be assigned, using the same pH60 probe, to one of the nine variable (V) gamma genes known to rearrange [(1987) EMBO J. 6, 1945-1950], our results show that a unique probe can detect all the TRG gamma rearrangements and be particularly useful for assessing the preferential usage of V gamma and J gamma segments in the TRG gamma-expressing cells.