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. 2018 Mar 7;12(1 Suppl):36-43.
doi: 10.2101/Ajid.12v1S.4. eCollection 2018.

ANTIVIRAL ACTIVITY OF Justicia gendarussa Burm.f. LEAVES AGAINST HIV-INFECTED MT-4 CELLS

Agustinus Widodo  1 , Prihartini Widiyanti  2   3 , Bambang Prajogo  4
Affiliations

ANTIVIRAL ACTIVITY OF Justicia gendarussa Burm.f. LEAVES AGAINST HIV-INFECTED MT-4 CELLS

Agustinus Widodo et al. Afr J Infect Dis. .

Abstract

Backgrounds: Justicia gendarussa Burm.f. has been known to have anti-HIV activity. This study was conducted to evaluate the effect of incubation time on the antiviral activity of the J. gendarussa leaves extract on HIV-infected MT-4 cells in vitro. Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the J. gendarussa leaves against HIV-1 reverse transcriptase receptor. It is expected that this research will provide scientific information on the development of J. gendarussa leaves as an anti-HIV drug.

Materials and methods: In the activity test, the effect of incubation time on the antiviral activity of J. gendarussa leaves on HIV-infected MT-4 cells were evaluated. During the activity test, a parameter of cytolysis effect inhibition on MT-4 cell line was observed after 4 days and 6 days incubation period. The molecular docking test is performed by using Molegro Virtual Docker software to determine the interaction of alkaloid and flavonoid compounds of J. gendarussa leaves with HIV-1 reverse transcriptase receptor.

Results: The incubation time influences the CC50 and EC50 value. Fractionated-70% ethanol extract of J. gendarussa leaves showed a higher anti-HIV activity with EC50 = 3.045 x 10-9 μg/mL, SI = 6.309 x 1012 (4 days of incubation) and EC50 = 6.066 μg/mL, SI = 58494.845 (6 days of incubation). From molecular docking test, it was found that flavonoid of J. gendarussa leaves could inhibit the activity of HIV reverse transcriptase enzyme.

Conclusion: Fractionated-70% ethanol extract of J. gendarussa has potential as an anti-HIV.

Keywords: Antiviral; HIV; Justicia gendarussa; MT-4 cell.

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Conflict of interest statement

Conflict of Interest: The authors declare that there are no conflicts of interest.

Figures

Figure 1
Figure 1
Effect of extracts of J. gendarussa leaves and ZDV/3TC on the viability of MT-4 cells after 4 days incubation period.
Figure 2
Figure 2
Effect of extracts of J. gendarussa leaves and ZDV/3TC on the viability of MT-4 cells after 6 days incubation period.
Figure 3
Figure 3
The Cytolysis inhibition effect of extracts of J. gendarussa leaves and ZDV/3TC on MT-4 cells viability were infected with HIV after 4 days incubation period.
Figure 4
Figure 4
The Cytolysis inhibition effect of extracts of J. gendarussa leaves and ZDV/3TC on MT-4 cells viability were infected with HIV after 6 days incubation period.
See this image and copyright information in PMC

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