Safety assessment of sodium acetate, sodium diacetate and potassium sorbate food additives

Hossein Mohammadzadeh-Aghdash; Yousef Sohrabi; Ali Mohammadi; Dariush Shanehbandi; Parvin Dehghan; Jafar Ezzati Nazhad Dolatabadi

doi:10.1016/j.foodchem.2018.03.020

Safety assessment of sodium acetate, sodium diacetate and potassium sorbate food additives

Food Chem. 2018 Aug 15:257:211-215. doi: 10.1016/j.foodchem.2018.03.020. Epub 2018 Mar 8.

Authors

Hossein Mohammadzadeh-Aghdash¹, Yousef Sohrabi¹, Ali Mohammadi², Dariush Shanehbandi², Parvin Dehghan³, Jafar Ezzati Nazhad Dolatabadi⁴

Affiliations

¹ Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
² Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
³ Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: dehghan.nut@gmail.com.
⁴ Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: ezzatij@tbzmed.ac.ir.

PMID: 29622200
DOI: 10.1016/j.foodchem.2018.03.020

Abstract

Cytotoxicity and genotoxicity of sodium acetate (SA), sodium diacetate (SDA), and potassium sorbate (PS) was tested on Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was investigated by MTT assay and flow cytometry analysis, while genotoxicity was evaluated using DNA fragmentation and DAPI staining assays. The growth of treated HUVECs with various concentrations of SA, SDA and PS decreased in a dose-and time-dependent manner. The IC50 of 487.71, 485.82 and 659.96 µM after 24 h and IC50 of 232.05, 190.19 and 123.95 µM after 48 h of treatment were attained for SA, SDA and PS, respectively. Flow cytometry analysis showed that early and late apoptosis percentage in treated cells was not considerable. Also neither considerable DNA fragmentation nor DNA smear was observed using DAPI staining and DNA ladder assays. Overall, it can be concluded that the aforementioned food additives can be used as safe additives at low concentration in food industry.

Keywords: Cytotoxicity; Food additives; Genotoxicity; HUVECs; Potassium sorbate; Sodium acetate; Sodium diacetate.

MeSH terms

Acetates / chemistry
Acetates / toxicity*
Cell Survival / drug effects
DNA Fragmentation / drug effects*
Food Additives / chemistry
Food Additives / toxicity*
Human Umbilical Vein Endothelial Cells
Humans
Microscopy, Fluorescence
Sodium Acetate / chemistry
Sodium Acetate / toxicity*
Sorbic Acid / chemistry
Sorbic Acid / toxicity*

Substances

Acetates
Food Additives
sodium diacetate
Sodium Acetate
Sorbic Acid