Accessory cell requirements for the mixed-leukocyte reaction and polyclonal mitogens, as studied with a new technique for enriching blood dendritic cells

Cell Immunol. 1988 Jan;111(1):167-82. doi: 10.1016/0008-8749(88)90061-5.

Abstract

Human blood dendritic cells can be enriched to 40-80% purity by a new technique that is simpler, provides greater yields than prior methods, and resolves other populations that are enriched in monocytes and B and T lymphocytes. The procedure involves separation over two Percoll gradients after 0 and 2 days of culture, followed by removal of contaminating monocytes by panning on plates coated with human Ig. The resultant dendritic cell-enriched fraction is 10 times or more potent than the monocyte-enriched populations in stimulating T-cell proliferative responses to alloantigens and to Con A. Small B lymphocytes are inactive in both systems. Dendritic cells do not initiate mitogenesis to anti-CD3 monoclonal antibodies, a response for which the monocyte appears to be the critical accessory cell.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigen-Presenting Cells / immunology*
  • Antigens, Differentiation, T-Lymphocyte / physiology
  • B-Lymphocytes / physiology
  • CD3 Complex
  • Cell Separation / methods
  • Centrifugation, Density Gradient
  • Concanavalin A / pharmacology
  • Dendritic Cells / cytology
  • Dendritic Cells / physiology*
  • Humans
  • In Vitro Techniques
  • Lymphocyte Culture Test, Mixed
  • Monocytes / physiology
  • Receptors, Fc / metabolism

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • Receptors, Fc
  • Concanavalin A