Advances and prospects in metabolic engineering of Zymomonas mobilis

Xia Wang; Qiaoning He; Yongfu Yang; Jingwen Wang; Katie Haning; Yun Hu; Bo Wu; Mingxiong He; Yaoping Zhang; Jie Bao; Lydia M Contreras; Shihui Yang

doi:10.1016/j.ymben.2018.04.001

Advances and prospects in metabolic engineering of Zymomonas mobilis

Metab Eng. 2018 Nov:50:57-73. doi: 10.1016/j.ymben.2018.04.001. Epub 2018 Apr 5.

Authors

Xia Wang¹, Qiaoning He², Yongfu Yang³, Jingwen Wang⁴, Katie Haning⁵, Yun Hu⁶, Bo Wu⁷, Mingxiong He⁸, Yaoping Zhang⁹, Jie Bao¹⁰, Lydia M Contreras¹¹, Shihui Yang¹²

Affiliations

¹ Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: xxwang@hubu.edu.cn.
² Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: Qiaoninghe@hubu.edu.cn.
³ Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: yongfu.yang@stu.hubu.edu.cn.
⁴ Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: jingwen.wang@stu.hubu.edu.cn.
⁵ Institute for Cellular and Molecular Biology, Department of Chemical Engineering, Cockrell School of Engineering, University of Texas at Austin, Austin, TX, United States. Electronic address: katie.haning@utexas.edu.
⁶ Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: huyun@hubu.edu.cn.
⁷ Key Laboratory of Development and Application of Rural Renewable Energy, Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, South Renmin Road, Chengdu 610041, China. Electronic address: wubo@caas.cn.
⁸ Key Laboratory of Development and Application of Rural Renewable Energy, Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, South Renmin Road, Chengdu 610041, China. Electronic address: hemingxiong@caas.cn.
⁹ DOE-Great Lakes Bioenergy Research Center (GLBRC), University of Wisconsin-Madison, Madison, WI, United States. Electronic address: yzhang8@wisc.edu.
¹⁰ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: jbao@ecust.edu.cn.
¹¹ Institute for Cellular and Molecular Biology, Department of Chemical Engineering, Cockrell School of Engineering, University of Texas at Austin, Austin, TX, United States. Electronic address: lcontrer@che.utexas.edu.
¹² Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China. Electronic address: Shihui.Yang@hubu.edu.cn.

PMID: 29627506
DOI: 10.1016/j.ymben.2018.04.001

Abstract

Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for commercial production of desirable bioproducts through metabolic engineering. In this review, we summarize the metabolic engineering progress achieved in Z. mobilis to expand its substrate and product ranges as well as to enhance its robustness against stressful conditions such as inhibitory compounds within the lignocellulosic hydrolysates and slurries. We also discuss a few metabolic engineering strategies that can be applied in Z. mobilis to further develop it as a robust workhorse for economic lignocellulosic bioproducts. In addition, we briefly review the progress of metabolic engineering in Z. mobilis related to the classical synthetic biology cycle of "Design-Build-Test-Learn", as well as the progress and potential to develop Z. mobilis as a model chassis for biorefinery practices in the synthetic biology era.

Keywords: Biochemical production; Biorefinery; Industrial chassis; Metabolic engineering; Synthetic biology; Zymomonas mobilis.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Lignin / genetics
Lignin / metabolism
Metabolic Engineering / methods*
Synthetic Biology / methods*
Zymomonas / genetics*
Zymomonas / metabolism*

Substances

lignocellulose
Lignin