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. 2015 Jun 6;1:6-10.
doi: 10.1016/j.biopen.2015.05.002. eCollection 2015.

Albumin Removal From Human Fibrinogen Preparations for Manufacturing Human Fibrin-Based Biomaterials

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Free PMC article

Albumin Removal From Human Fibrinogen Preparations for Manufacturing Human Fibrin-Based Biomaterials

Vaibhav Sharma et al. Biochim Open. .
Free PMC article

Abstract

Commercially available two component human fibrin sealants are commonly used to manufacture human fibrin-based biomaterials. However, this method is costly and allows little room for further tuning of the biomaterial. Human fibrinogen solutions offer a more cost-effective and versatile alternative to manufacture human fibrin-based biomaterials. Yet, human fibrinogen is highly unstable and contains certain impurities like human albumin. Within the context of biomaterials and tissue engineering we offer a simple yet novel solution based on classical biochemical techniques to significantly reduce albumin in human fibrinogen solutions. This method can be used for various tissue engineering and biomedical applications as an initial step in the manufacturing of human fibrin-based biomaterials to optimise their regenerative application.

Keywords: Albumin; Biomaterials; Dialysis; Human fibrin; Human fibrinogen.

Figures

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Fig. 1
Fig. 1
Graphical summary of the method proposed in this paper for removal of albumin from human fibrinogen preparations for manufacturing human fibrin-based biomaterials.
Fig. 2
Fig. 2
Top left, SDS-PAGE gel. Top right, BSA standard curve obtained from the SDS-PAGE gel by image analysis. Bottom right, table showing amount (µg) and percentage of albumin in CSL product before and after the dialysis. Bottom left, coagulation kinetic assay showing functionality of dialysed human fibrinogen.

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References

    1. Amrani D.L., Diorio J.P., Delmotte Y. Wound healing. Role of commercial fibrin sealants. Ann. N. Y. Acad. Sci. 2001;936:566–579. - PubMed
    1. Shaikh F.M., Callanan A., Kavanagh E.G., Burke P.E., Grace P.A., McGloughlin T.M. Fibrin: a natural biodegradable scaffold in vascular tissue engineering. Cells. Tissues. Organs. 2008;188:333–346. - PubMed
    1. Jackson M.R. Fibrin sealants in surgical practice: an overview. Am. J. Surg. 2001;182:1S–7S. - PubMed
    1. Bensaïd W., Triffitt J.T., Blanchat C., Oudina K., Sedel L., Petite H. A biodegradable fibrin scaffold for mesenchymal stem cell transplantation. Biomaterials. 2003;24:2497–2502. - PubMed
    1. Ye Q., Zünd G., Benedikt P., Jockenhoevel S., Hoerstrup S.P., Sakyama S. Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering. Eur. J. Cardio-Thoracic Surg. 2000;17:587–591. - PubMed

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