Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing

Methods Mol Biol. 2018;1771:83-95. doi: 10.1007/978-1-4939-7792-5_7.

Abstract

Biomolecule microarrays are generally produced by conventional microarrayer, i.e., by contact or inkjet printing. Microcontact printing represents an alternative way of deposition of biomolecules on solid supports but even if various biomolecules have been successfully microcontact printed, the production of biomolecule microarrays in routine by microcontact printing remains a challenging task and needs an effective, fast, robust, and low-cost automation process. Here, we describe the production of biomolecule microarrays composed of extracellular matrix protein for the fabrication of cell microarrays by using an automated microcontact printing device. Large scale cell microarrays can be reproducibly obtained by this method.

Keywords: Biomolecule microarrays; Cell architecture; Cell microarrays; Microcontact printing; Micropatterning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques
  • Coated Materials, Biocompatible
  • Extracellular Matrix Proteins
  • Printing, Three-Dimensional*
  • Tissue Array Analysis / instrumentation
  • Tissue Array Analysis / methods*

Substances

  • Coated Materials, Biocompatible
  • Extracellular Matrix Proteins