Cytokine induced killer cells-assisted delivery of chlorin e6 mediated self-assembled gold nanoclusters to tumors for imaging and immuno-photodynamic therapy

Fangfang Xia; Wenxiu Hou; Yanlei Liu; Wentao Wang; Yu Han; Meng Yang; Xiao Zhi; Chenlu Li; Daizong Qi; Tianliang Li; Jesus Martinez de la Fuente; Chunlei Zhang; Jie Song; Daxiang Cui

doi:10.1016/j.biomaterials.2018.03.048

Cytokine induced killer cells-assisted delivery of chlorin e6 mediated self-assembled gold nanoclusters to tumors for imaging and immuno-photodynamic therapy

Biomaterials. 2018 Jul:170:1-11. doi: 10.1016/j.biomaterials.2018.03.048. Epub 2018 Mar 28.

Authors

Affiliations

¹ Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Centre for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, PR China.
² School of Materials and Chemical Engineering, Chongqing University of Arts and Sciences, Yongchuan, Chongqing 402160, PR China.
³ National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China; Instituto de Ciencia de Materiales, CSIC/University of Zaragoza and CIBER-BBN, Zaragoza 50018, Spain.
⁴ Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Centre for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, PR China; National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. Electronic address: sjie@sjtu.edu.cn.
⁵ Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Centre for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, PR China; National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. Electronic address: dxcui@sjtu.edu.cn.

PMID: 29635107
DOI: 10.1016/j.biomaterials.2018.03.048

Abstract

The cytotoxicity and unique tumor-tropic properties of cytokine-induced killer (CIK) cells render them promising in the field of cancer immunotherapy and delivery systems. Here, we report a novel and facile approach to assemble gold nanoclusters (GNCs) into stable and monodispersed nanoparticles (NPs) using Chlorin e6 (Ce6) molecules. Notably, the fluorescence intensity of the GNCs-Ce6 NPs was about 4.5 folds stronger than the GNCs counterparts. The as-prepared GNCs-Ce6 NPs were conjugated with CD3 antibody (Ab) and further employed to label CIK cells to create a CIK cell-based drug delivery system (Ce6-GNCs-Ab-CIK). The Ce6-GNCs-Ab-CIK exhibited high tumor-targeting efficiency and excellent therapeutic efficacy toward MGC-803 tumor-bearing mice. Benefiting from the synergistic therapeutic effect between GNCs-Ce6-Ab NPs and CIK cells, the GNCs-Ce6-Ab-CIK strategy may present an ideal cancer theranostic platform for tumor targeted imaging and combination therapy.

Keywords: CIK cells; Chlorin e6; Gold nanoclusters; Immunotherapy; Near-infrared fluorescence imaging; Photodynamic therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies / pharmacology
Cell Line, Tumor
Chlorophyllides
Cytokine-Induced Killer Cells / drug effects
Cytokine-Induced Killer Cells / metabolism*
Cytokines / metabolism
Drug Delivery Systems
Endocytosis / drug effects
Gold / chemistry*
Humans
Immunotherapy*
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Mice, Inbred C57BL
Mice, Nude
Neoplasms / drug therapy*
Neoplasms / immunology*
Neoplasms / pathology
Phenotype
Photochemotherapy*
Porphyrins / pharmacology*

Substances

Antibodies
Chlorophyllides
Cytokines
Porphyrins
phytochlorin
Gold