Reversible Redox Activity by Ion-pH Dually Modulated Duplex Formation of i-Motif DNA with Complementary G-DNA

Soyoung Chang; Tugba Kilic; Chang Kee Lee; Huseyin Avci; Hojae Bae; Shirin Mesbah Oskui; Sung Mi Jung; Su Ryon Shin; Seon Jeong Kim

doi:10.3390/nano8040226

Reversible Redox Activity by Ion-pH Dually Modulated Duplex Formation of i-Motif DNA with Complementary G-DNA

Nanomaterials (Basel). 2018 Apr 8;8(4):226. doi: 10.3390/nano8040226.

Authors

Soyoung Chang¹, Tugba Kilic^{2

3

4}, Chang Kee Lee⁵, Huseyin Avci^{6

7

8}, Hojae Bae⁹, Shirin Mesbah Oskui^{10

11}, Sung Mi Jung¹², Su Ryon Shin^{13

14

15}, Seon Jeong Kim¹⁶

Affiliations

¹ Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, Seoul 04763, Korea. ssoii88@kaist.ac.kr.
² Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. tugba.kilic@epfl.ch.
³ Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. tugba.kilic@epfl.ch.
⁴ Department of Biomedical Engineering, Izmir Katip Celebi University, Izmir 35620, Turkey. tugba.kilic@epfl.ch.
⁵ Korea Packaging Center, Korea Institute of Industrial Technology, Bucheon 14449, Korea. withs@kitech.re.kr.
⁶ Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. huseyin.avci44@gmail.com.
⁷ Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. huseyin.avci44@gmail.com.
⁸ Department of Metallurgical and Materials Engineering, Eskisehir Osmangazi University, Eskisehir 26040, Turkey. huseyin.avci44@gmail.com.
⁹ KU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, Korea. hojaebae@gmail.com.
¹⁰ Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. shiriinn@gmail.com.
¹¹ Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. shiriinn@gmail.com.
¹² Future Environmental Research Center, Korea Institute of Toxicology, Jinju 52834, Korea. sungmijung@gmail.com.
¹³ Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, Seoul 04763, Korea. sshin4@bwh.harvard.edu.
¹⁴ Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. sshin4@bwh.harvard.edu.
¹⁵ Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. sshin4@bwh.harvard.edu.
¹⁶ Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, Seoul 04763, Korea. sjk@hanyang.ac.kr.

Abstract

The unique biological features of supramolecular DNA have led to an increasing interest in biomedical applications such as biosensors. We have developed an i-motif and G-rich DNA conjugated single-walled carbon nanotube hybrid materials, which shows reversible conformational switching upon external stimuli such as pH (5 and 8) and presence of ions (Li⁺ and K⁺). We observed reversible electrochemical redox activity upon external stimuli in a quick and robust manner. Given the ease and the robustness of this method, we believe that pH- and ion-driven reversible DNA structure transformations will be utilized for future applications for developing novel biosensors.

Keywords: DNA; biosensor; carbon nanotube.