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. 2018 Apr 10;19(4):1127.
doi: 10.3390/ijms19041127.

In Vitro and In Vivo Osteogenic Activity of Titanium Implants Coated by Pulsed Laser Deposition With a Thin Film of Fluoridated Hydroxyapatite

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Free PMC article

In Vitro and In Vivo Osteogenic Activity of Titanium Implants Coated by Pulsed Laser Deposition With a Thin Film of Fluoridated Hydroxyapatite

Luyuan Chen et al. Int J Mol Sci. .
Free PMC article

Abstract

To enhance biocompatibility, osteogenesis, and osseointegration, we coated titanium implants, by krypton fluoride (KrF) pulsed laser deposition, with a thin film of fluoridated hydroxyapatite (FHA). Coating was confirmed by scanning electron microscopy (SEM) and scanning probe microscopy (SPM), while physicochemical properties were evaluated by attenuated reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Calcium deposition, osteocalcin production, and expression of osteoblast genes were significantly higher in rat bone marrow mesenchymal stem cells seeded on FHA-coated titanium than in cells seeded on uncoated titanium. Implantation into rat femurs also showed that the FHA-coated material had superior osteoinductive and osseointegration activity in comparison with that of traditional implants, as assessed by microcomputed tomography and histology. Thus, titanium coated with FHA holds promise as a dental implant material.

Keywords: fluoridated hydroxyapatite; implant; osteogenic activity; pulsed laser deposition; rat femur model.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Gross appearance of Titanium (Ti) and fluoridated hydroxyapatite (FHA)-coated Ti (a) discs and (b) screws.
Figure 2
Figure 2
Scanning electron micrographs of (a,b) Ti and (c,d) FHA-coated screws.
Figure 3
Figure 3
Scanning probe micrographs of (a) Ti and (b) FHA-coated surfaces.
Figure 4
Figure 4
Fourier transform infrared spectra at (a) 1200–400 cm−1 and (b) 3600–3000 cm−1.
Figure 5
Figure 5
Expression of (a) ALP, (b) RUNX2, and (c) BMP in cells seeded on Ti and FHA-coated discs. * p < 0.05.
Figure 6
Figure 6
(a) Calcium deposition and (b) osteocalcin production in cells seeded on Ti and FHA-coated discs. * p < 0.05.
Figure 7
Figure 7
Implantation into rat femurs. (a) Incision; (b) Drilling of a hole; (c) Placement of implant; (d) Closure.
Figure 8
Figure 8
Transverse reconstructed microcomputed tomographs of (a,c) Ti and (b,d) FHA-coated implants after (a,b) 4 weeks and (c,d) 8 weeks.
Figure 9
Figure 9
(a,e) Bone volume to total volume ratio (BV/TV), (b,f) mean trabecular number (Tb.N), (c,g) mean trabecular separation (Tb.Sp), and (d,h) mean trabecular thickness (Tb.Th) around implants after (ad) 4 weeks (4 w) and (eh) 8 weeks (8 w). * p < 0.05.
Figure 10
Figure 10
Villanueva staining of bone tissues around (a) Ti and (b) FHA-coated implants.
Figure 11
Figure 11
(a) Bone area ratio (BA) and (b) bone–implant contact (BIC) in Ti and FHA-coated implants. * p < 0.05.
Figure 12
Figure 12
Fluorescent labeling of new bone and mineralization around (a) Ti and (b) FHA-coated implants.
Figure 13
Figure 13
Fluorescently labeled bone area (LBA) after (a) 1 week, (b) 4 weeks, and (c) 8 weeks. * p < 0.05.

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