Structures of the Mycobacterium Tuberculosis GlpX Protein (Class II fructose-1,6-bisphosphatase): Implications for the Active Oligomeric State, Catalytic Mechanism and Citrate Inhibition

Acta Crystallogr D Struct Biol. 2018 Apr 1;74(Pt 4):321-331. doi: 10.1107/S2059798318002838. Epub 2018 Apr 3.

Abstract

The crystal structures of native class II fructose-1,6-bisphosphatase (FBPaseII) from Mycobacterium tuberculosis at 2.6 Å resolution and two active-site protein variants are presented. The variants were complexed with the reaction product fructose 6-phosphate (F6P). The Thr84Ala mutant is inactive, while the Thr84Ser mutant has a lower catalytic activity. The structures reveal the presence of a 222 tetramer, similar to those described for fructose-1,6/sedoheptulose-1,7-bisphosphatase from Synechocystis (strain 6803) as well as the equivalent enzyme from Thermosynechococcus elongatus. This homotetramer corresponds to a homologous oligomer that is present but not described in the crystal structure of FBPaseII from Escherichia coli and is probably conserved in all FBPaseIIs. The constellation of amino-acid residues in the active site of FBPaseII from M. tuberculosis (MtFBPaseII) is conserved and is analogous to that described previously for the E. coli enzyme. Moreover, the structure of the active site of the partially active (Thr84Ser) variant and the analysis of the kinetics are consistent with the previously proposed catalytic mechanism. The presence of metabolites in the crystallization medium (for example citrate and malonate) and in the corresponding crystal structures of MtFBPaseII, combined with their observed inhibitory effect, could suggest the existence of an uncharacterized inhibition of this class of enzymes besides the allosteric inhibition by adenosine monophosphate observed for the Synechocystis enzyme. The structural and functional insights derived from the structure of MtFBPaseII will provide critical information for the design of lead inhibitors, which will be used to validate this target for future chemical intervention.

Keywords: GlpX; Mycobacterium tuberculosis; TCA cycle intermediates; allosteric regulation; class II fructose-1,6-bisphosphatase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Allosteric Regulation*
  • Catalysis
  • Catalytic Domain
  • Citrates / antagonists & inhibitors*
  • Enzyme Inhibitors
  • Escherichia coli Proteins
  • Fructose-Bisphosphatase / chemistry*
  • Fructose-Bisphosphatase / genetics
  • Kinetics
  • Mutant Proteins / chemistry
  • Mutation
  • Mycobacterium tuberculosis / enzymology*
  • Protein Multimerization
  • Synechocystis / chemistry

Substances

  • Citrates
  • Enzyme Inhibitors
  • Escherichia coli Proteins
  • Mutant Proteins
  • Fructose-Bisphosphatase