Metabolite Depletion Affects Flux Profiling of Cell Lines

Trends Biochem Sci. 2018 Jun;43(6):395-397. doi: 10.1016/j.tibs.2018.03.009. Epub 2018 Apr 12.


Quantifying the rate of consumption and release of metabolites (i.e., flux profiling) has become integral to the study of cancer. The fluxes as well as the growth of the cells may be affected by metabolite depletion during cultivation.

Keywords: cancer; glutamine; metabolic modeling; serine.

Publication types

  • Review

MeSH terms

  • Cell Culture Techniques
  • Humans
  • Neoplasms / metabolism*
  • Neoplasms / pathology*
  • Tumor Cells, Cultured