A new method of inhibiting glycolysis in blood samples

Clin Chim Acta. 1988 Feb 29;172(1):101-8. doi: 10.1016/0009-8981(88)90125-8.

Abstract

The maintenance of hydrogen ion concentration in blood samples at pH 5.3-5.9 immediately inhibits glycolysis. This effect is due to the inhibition of all glycolytic enzymes, as shown by measurement of various glycolytic intermediates. At the inhibitory pH at 25 degrees C, the glucose content did not decrease over a period of 8 h, but it did decrease by 1.3 +/- 1.1 mg/dl over 24 h.

MeSH terms

  • Adenosine Triphosphate / blood
  • Blood Glucose / analysis
  • Blood Specimen Collection
  • Electrolytes / blood
  • Erythrocytes / metabolism
  • Glycolysis / drug effects*
  • Hexokinase / blood
  • Humans
  • Hydrogen-Ion Concentration
  • Phosphofructokinase-1 / blood

Substances

  • Blood Glucose
  • Electrolytes
  • Adenosine Triphosphate
  • Hexokinase
  • Phosphofructokinase-1