Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 18 (1), 131

Anti-osteoarthritic Effects of ChondroT in a Rat Model of Collagenase-Induced Osteoarthritis

Affiliations

Anti-osteoarthritic Effects of ChondroT in a Rat Model of Collagenase-Induced Osteoarthritis

Jiwon Jeong et al. BMC Complement Altern Med.

Abstract

Background: Previously, we reported that ChondorT showed significant anti-arthritis and anti-inflammatory effects. ChondroT, a new herbal medication, consists of the water extracts of Osterici Radix, Lonicerae Folium, Angelicae Gigantis Radix, Clematidis Radix, and Phellodendri Cortex (6:4:4:4:3). The objective of this study was to investigate the effects of ChondroT in collagenase-induced osteoarthritis rat model.

Methods: Osteoarthritis was induced by the injection of collagenase into the right knee joint cavity of rats. The samples were divided into seven groups [intact (n = 6), control (n = 6), indomethacin (n = 6), Joins tab (n = 6), ChondroT50 (n = 6), ChondroT100 (n = 6), and ChondroT200 (n = 6)]. The control group was administered normal saline, indomethacin group was administered indomethacin (2 mg/kg), and Joins tab group was administered Joins Tab (20 mg/kg). The ChondroT50, ChondroT100, and ChondroT200 groups were administered 50, 100, and 200 mg/kg of ChondroT, respectively. All oral administrations were initiated 7 days after the induction of arthritis and were continued for a total of 12 days. At the end of the experiment, serum aminotransferase, albumin, blood urea nitrogen, creatinine, leukocyte, and inflammatory cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] were analyzed. Hematoxylin and eosin (H&E) and safranin O-fast green staining of the articular structures of the knee joint were performed.

Results: TNF-α and IL-1β decreased in the ChondroT100 and ChondroT200 groups compared with those in the control group. IL-6 and aspartate aminotransferase decreased in the ChondroT50, ChondroT100, and ChondroT200 groups compared with that in the control group. Albumin, WBC and lymphocytes decreased in the ChondroT100 and ChondroT200 groups compared with those in the control group. In H&E stain, synoviocytes, cartilage lacunae, and chondrocytes were well preserved in the ChondroT100 and ChondroT200 groups, and safranin O-fast staining showed a clear reaction of proteoglycans in the ChondroT100 and ChondroT200 groups.

Conclusions: Based on these results, it can be proposed that ChondroT has anti-osteoarthritic effects on collagenase-induced rat model.

Keywords: ChondroT; Collagenase; Inflammatory cytokine; Osteoarthritis.

Conflict of interest statement

Ethics approval

All animal experiments were conducted in accordance with the Institutional Animal Care and Use Committee (IACUC) guidelines. All the animal procedures were carried out in accordance with the guidelines of the Animal Care and Use Committee of Dongshin University. The animal study was submitted to, and approved by Animal Care and Use Committee of Dongshin University (Permission number: DSU-2015-1003-01).

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
a Chemical structures of compounds 1–3; b HPLC chromatogram of a standard solution and ChondroT, with detection at 330 nm. (1) Chlorogenic acid, (2) berberine Cl, and (3) decursin
Fig. 2
Fig. 2
Effect of ChondroT on TNF-α, IL-1β, and IL-6 levels in rats with collagenase -induced arthritis
Fig. 3
Fig. 3
Histopathological changes (H&E stain) in the knee joint after ChondroT administration in collagenase induced arthritis rats. In H&E stain, synoviocytes, cartilage lacunae, and chondrocytes were well preserved in the ChondroT100 and ChondroT200 groups. HE-stain, Scale bars = 500 μm. a Intact, b Control, c Indomethacin, d Joins Tab, e ChondroT50, f ChondroT100, g ChondroT200
Fig. 4
Fig. 4
Histopathological changes (Safranin O-fast staining) in the knee joint after ChondroT administration in collagenase-induced arthritis rats. Safranin O-fast staining showed a clear reaction of proteoglycans in ChondroT200 group. Safranin O-fast stain, Scale bars = 100 μm. a Intact, b Control, c Indomethacin, d Joins Tab, e ChondroT50, f ChondroT100, g ChondroT200
Fig. 5
Fig. 5
Changes in the blood WBC, neutrophils, lymphocytes, monocytes, and eosinophils contents after ChondroT administration in collagenase-induced arthritis rats
Fig. 6
Fig. 6
Changes in serum aminotransferase content after ChondroT administration in collagenase-induced arthritis rats
Fig. 7
Fig. 7
Changes in serum albumin, BUN, and creatinine content after ChondroT administration in collagenase-induced arthritis rats

Similar articles

See all similar articles

Cited by 3 articles

References

    1. Cross M, Smith E, Hoy D, Nolte S, Ackerman I, Fransen M, et al. The global burden of hip and knee osteoarthritis: estimates from the global burden of disease 2010 study. Ann Rheum Dis. 2014;73:1323–1330. doi: 10.1136/annrheumdis-2013-204763. - DOI - PubMed
    1. Kim JH, Ryu KH, Jung KW, Han CK, Kwak WJ, Cho YB. Effects of SKI306X on arachidonate metabolism and other inflammatory mediators. Biol Pharm Bull. 2005;28:1615–1620. doi: 10.1248/bpb.28.1615. - DOI - PubMed
    1. Vervoordeldonk MJ, Tak PP. Cytokines in rheumatoid arthritis. Curr Rheumatol Rep. 2002;4:208–217. doi: 10.1007/s11926-002-0067-0. - DOI - PubMed
    1. Nishimoto N. Interleukin-6 in rheumatoid arthritis. Curr Opin Rheumatol. 2006;18:277–281. doi: 10.1097/01.bor.0000218949.19860.d1. - DOI - PubMed
    1. Zeng L, Wang W, Rong XF, Zhong Y, Jia P, Zhou GQ. Chondroprotective effects and multi-target mechanisms of icariin in IL-1 beta-induced human SW 1353 chondrosarcoma cells and a rat osteoarthritis model. Int Immunopharmacol. 2014;18:175–181. doi: 10.1016/j.intimp.2013.11.021. - DOI - PubMed
Feedback