Long-term evaluation of the stability of dentin matrix following treatments with aqueous solutions of titanium tetrafluoride at different concentrations

Arch Oral Biol. 2018 Jul:91:51-56. doi: 10.1016/j.archoralbio.2018.04.006. Epub 2018 Apr 10.


Objective: The purpose of this study was to investigate the effects of aqueous solutions of different concentrations of titanium tetrafluoride (TiF4) on dentin matrix stability up to six months.

Design: Dentin specimens prepared from fifteen nonerupted molars were demineralized and randomly distributed into groups: 2.5% TiF4, 4% TiF4, 1000 ppm NaF, and control (distilled water). The modulus of elasticity (ME) and dry masses of the dentin matrix were determined at baseline, and up to 6 months following treatment. Collagen solubilization was estimated by hydroxyproline (HYP) quantification in the simulated body fluid used to store the dentin specimens.

Results: The 2.5% TiF4 group presented higher ME immediately after treatment, and at 3 and 6 months storage, whereas 4% TiF4 showed higher means at 3 and 6 months (p < 0.001). No significant differences were observed among the groups over time (p = 0.9325). However, the 2.5% TiF4 group showed significantly higher ME than the control group, immediately after treatment. All the groups presented significantly higher mass change immediately, compared with 3 and 6 months (p < 0.0001). Except for the 4% TiF4 group, HYP release was higher in the first quarter (p = 0.0152), when no significant differences were found among the groups. In the second quarter, the means were significantly higher in the 2.5% TiF4 and 4% TiF4 groups. The group treated with 2.5% TiF4 had a statistically higher HYP release than the control group.

Conclusion: An aqueous solution of 2.5% TiF4 increases the immediate stiffness values, but does not stabilize the collagenous dentin matrix.

Keywords: Dentin matrix; Elastic modulus; Hydroxyproline; Stiffness; Titanium tetrafluoride.

MeSH terms

  • Cariostatic Agents / pharmacology
  • Dentin / chemistry*
  • Dentin / drug effects*
  • Elastic Modulus / drug effects
  • Fluorides / administration & dosage*
  • Fluorides / pharmacology*
  • Humans
  • Hydroxyproline
  • In Vitro Techniques
  • Materials Testing
  • Molar / chemistry
  • Molar / drug effects
  • Peptide Hydrolases / drug effects
  • Time Factors
  • Titanium / administration & dosage*
  • Titanium / pharmacology*
  • Tooth Demineralization / drug therapy


  • Cariostatic Agents
  • Titanium
  • Peptide Hydrolases
  • Fluorides
  • Hydroxyproline
  • titanium tetrafluoride