Photodynamic inactivation assisted by localized surface plasmon resonance of silver nanoparticles: In vitro evaluation on Escherichia coli and Streptococcus mutans

Photodiagnosis Photodyn Ther. 2018 Jun;22:191-196. doi: 10.1016/j.pdpdt.2018.04.007. Epub 2018 Apr 17.

Abstract

Localized surface plasmon resonance (LSPR) of gold nanoparticles has been reported to increase the antimicrobial effect of the photodynamic therapy. Although silver nanoparticles (AgNPs) are an efficient growth inhibitor of microorganisms, no studies exploring LSPR of AgNPs to enhance the photodynamic inactivation (PDI) have been related. In this work, we described the LSPR phenomenon of AgNP sand investigated its interaction with riboflavin, a natural photosensitizer. We evaluated the use of AgNPs coated with pectin (p-AgNP) in riboflavin (Rb)-mediated PDI of Escherichia coli (Gram- bacteria) and Streptococcus mutans (Gram + bacteria) using a blue light-emitting diode (λ = 455 ± 20 nm) of optical power 200 mW. Irradiance was 90 mW/cm2 and radiant exposure varied according to the time exposure. Uptake of Rb and p-AgNP by the cells was evaluated by measuring the supernatant absorption spectra of the samples. We observed that LSPR of p-AgNPs was able to enhance the riboflavin photodynamic action on S. mutans but not on E. coli, probably due to the lower uptake of Rb by E. coli. Taken together, our results provide insights to explore the use of the LPRS promoted by silver nanostructures to optimize antimicrobial PDI protocols.

Keywords: Antimicrobial; Bacterial inactivation; Blue LED; Metallic nanoparticle; Photosensitizer uptake.

MeSH terms

  • Escherichia coli / drug effects*
  • Metal Nanoparticles / chemistry*
  • Pectins / chemistry
  • Photochemotherapy / methods
  • Photosensitizing Agents / pharmacology*
  • Riboflavin / pharmacology*
  • Silver / chemistry*
  • Streptococcus mutans / drug effects*
  • Surface Plasmon Resonance / methods

Substances

  • Photosensitizing Agents
  • Silver
  • Pectins
  • Riboflavin