Nucleus accumbens controls wakefulness by a subpopulation of neurons expressing dopamine D 1 receptors

Nat Commun. 2018 Apr 20;9(1):1576. doi: 10.1038/s41467-018-03889-3.

Abstract

Nucleus accumbens (NAc) is involved in behaviors that depend on heightened wakefulness, but its impact on arousal remains unclear. Here, we demonstrate that NAc dopamine D1 receptor (D1R)-expressing neurons are essential for behavioral arousal. Using in vivo fiber photometry in mice, we find arousal-dependent increases in population activity of NAc D1R neurons. Optogenetic activation of NAc D1R neurons induces immediate transitions from non-rapid eye movement sleep to wakefulness, and chemogenetic stimulation prolongs arousal, with decreased food intake. Patch-clamp, tracing, immunohistochemistry, and electron microscopy reveal that NAc D1R neurons project to the midbrain and lateral hypothalamus, and might disinhibit midbrain dopamine neurons and lateral hypothalamus orexin neurons. Photoactivation of terminals in the midbrain and lateral hypothalamus is sufficient to induce wakefulness. Silencing of NAc D1R neurons suppresses arousal, with increased nest-building behaviors. Collectively, our data indicate that NAc D1R neuron circuits are essential for the induction and maintenance of wakefulness.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Circadian Rhythm / physiology
  • Dopaminergic Neurons / metabolism*
  • Hypothalamic Area, Lateral / physiology*
  • Male
  • Mesencephalon / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Nucleus Accumbens / metabolism*
  • Patch-Clamp Techniques
  • Photometry / methods
  • Receptors, Dopamine D1 / biosynthesis
  • Receptors, Dopamine D1 / metabolism*
  • Sleep / physiology
  • Wakefulness / physiology*

Substances

  • Drd1 protein, mouse
  • Receptors, Dopamine D1