Colloidal gold-based immunochromatographic strip assay for the rapid detection of three natural estrogens in milk

Zhongxing Wang; Lingling Guo; Liqiang Liu; Hua Kuang; Chuanlai Xu

doi:10.1016/j.foodchem.2018.03.087

Colloidal gold-based immunochromatographic strip assay for the rapid detection of three natural estrogens in milk

Food Chem. 2018 Sep 1:259:122-129. doi: 10.1016/j.foodchem.2018.03.087. Epub 2018 Mar 21.

Authors

Zhongxing Wang¹, Lingling Guo¹, Liqiang Liu¹, Hua Kuang², Chuanlai Xu³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address: kuangh@jiangnan.edu.cn.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address: xcl@jiangnan.edu.cn.

PMID: 29680034
DOI: 10.1016/j.foodchem.2018.03.087

Abstract

In this study, we developed highly sensitive and specific monoclonal antibodies (mAbs) against estrone (E₁), 17β-estradiol (17β-E₂), and estriol (E₃). The half-maximal inhibitory concentration values of anti-E₁, anti-17β-E₂, and anti-E₃ mAbs were 0.46, 0.36, and 0.39 ng/mL, respectively, based on competitive enzyme-linked immunosorbent assay (ic-ELISA) results. A rapid colloidal gold-based immunoassay strip assay was developed for the determination of E_1, 17β-E₂, and E₃ residues in milk samples. The assay had a visual cut-off value of 5 ng/mL, and required 10 min to assess with the naked eye. The results obtained from the immunochromatographic strip assay were consistent with those obtained from ic-ELISA and gas chromatography-mass spectrometry. The immunochromatographic strip assay is useful and rapid for the detection of E₁, 17β-E₂, and E₃ in milk.

Keywords: 17β-estradiol; Estriol; Estrone; Immunochromatographic strip; Monoclonal antibody.

MeSH terms

Animals
Antibodies, Monoclonal
Chromatography, Affinity / methods*
Estradiol / analysis
Estradiol / immunology
Estriol / analysis
Estriol / immunology
Estrogens / analysis*
Estrogens / immunology
Estrone / analysis
Estrone / immunology
Female
Food Analysis / methods*
Gold Colloid / chemistry
Mice, Inbred BALB C
Milk / chemistry*

Substances

Antibodies, Monoclonal
Estrogens
Gold Colloid
Estrone
Estradiol
Estriol