Reactivity and Specificity of RNase T 1, RNase A, and RNase H toward Oligonucleotides of RNA Containing 8-Oxo-7,8-dihydroguanosine

Biochemistry. 2018 May 22;57(20):2971-2983. doi: 10.1021/acs.biochem.8b00277. Epub 2018 May 4.

Abstract

Understanding how oxidatively damaged RNA interacts with ribonucleases is important because of its proposed role in the development and progression of disease. Thus, understanding structural aspects of RNA containing lesions generated under oxidative stress, as well as its interactions with other biopolymers, is fundamental. We explored the reactivity of RNase A, RNase T1, and RNase H toward oligonucleotides of RNA containing 8-oxo-7,8-dihydroguanosine (8oxoG). This is the first example that addresses this relationship and will be useful for understanding (1) how these RNases can be used to characterize the structural impact that this lesion has on RNA and (2) how oxidatively modified RNA may be handled intracellularly. 8-OxoG was incorporated into 10-16-mers of RNA, and its reactivity with each ribonuclease was assessed via electrophoretic analyses, circular dichroism, and the use of other C8-purine-modified analogues (8-bromoguanosine, 8-methoxyguanosine, and 8-oxoadenosine). RNase T1 does not recognize sites containing 8-oxoG, while RNase A recognizes and cleaves RNA at positions containing this lesion while differentiating if it is involved in H-bonding. The selectivity of RNase A followed the order C > 8-oxoG ≈ U. In addition, isothermal titration calorimetry showed that an 8-oxoG-C3'-methylphosphate derivative can inhibit RNase A activity. Cleavage patterns obtained from RNase H displayed changes in reactivity in a sequence- and concentration-dependent manner and displayed recognition at sites containing the modification in some cases. These data will aid in understanding how this modification affects reactivity with ribonucleases and will enable the characterization of global and local structural changes in oxidatively damaged RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circular Dichroism
  • Guanosine / analogs & derivatives
  • Guanosine / chemistry
  • Guanosine / genetics
  • Humans
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics*
  • Oxidative Stress / genetics
  • RNA / chemistry
  • RNA / genetics
  • Ribonuclease H / chemistry
  • Ribonuclease H / genetics*
  • Ribonuclease T1 / chemistry
  • Ribonuclease T1 / genetics*
  • Ribonuclease, Pancreatic / chemistry
  • Ribonuclease, Pancreatic / genetics*
  • Ribonucleases / chemistry
  • Ribonucleases / genetics
  • Substrate Specificity

Substances

  • Oligonucleotides
  • Guanosine
  • 8-hydroxyguanosine
  • RNA
  • Ribonucleases
  • Ribonuclease H
  • Ribonuclease T1
  • Ribonuclease, Pancreatic