Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells

Methods Mol Biol. 2018:1773:73-91. doi: 10.1007/978-1-4939-7799-4_7.

Abstract

Murine models of allograft transplantation are valuable for understanding the immunological mechanisms of allograft acceptance and rejection, the evaluation of immunosuppressive drugs and strategies, and the restoration of functional defects. Herein, we describe methods to create a skin murine allograft surgical model and how to administer adipose-derived stromal cells (ASC) with limited numbers of donor bone marrow to create stable multilineage donor cell chimerism and indefinite immunological tolerance.

Keywords: Adipose stem cells; Donor cell chimerism; In vivo immune regulation; Skin allograft transplantation; Tolerance induction.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Allografts / immunology
  • Animals
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / immunology
  • Female
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Models, Animal
  • Skin Transplantation*
  • Transplantation Chimera / immunology*
  • Transplantation Tolerance / immunology*
  • Transplantation, Homologous