Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells

Anthony D Foster; Nicholas Clark; Thomas A Davis

doi:10.1007/978-1-4939-7799-4_7

Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells

Methods Mol Biol. 2018:1773:73-91. doi: 10.1007/978-1-4939-7799-4_7.

Authors

Anthony D Foster^{1

2}, Nicholas Clark¹, Thomas A Davis^{3

4}

Affiliations

¹ Regenerative Medicine Department, Naval Medical Research Center, Silver Spring, MD, USA.
² Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
³ Regenerative Medicine Department, Naval Medical Research Center, Silver Spring, MD, USA. thomas.davis@usuhs.edu.
⁴ Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. thomas.davis@usuhs.edu.

PMID: 29687382
DOI: 10.1007/978-1-4939-7799-4_7

Abstract

Murine models of allograft transplantation are valuable for understanding the immunological mechanisms of allograft acceptance and rejection, the evaluation of immunosuppressive drugs and strategies, and the restoration of functional defects. Herein, we describe methods to create a skin murine allograft surgical model and how to administer adipose-derived stromal cells (ASC) with limited numbers of donor bone marrow to create stable multilineage donor cell chimerism and indefinite immunological tolerance.

Keywords: Adipose stem cells; Donor cell chimerism; In vivo immune regulation; Skin allograft transplantation; Tolerance induction.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Allografts / immunology
Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / immunology
Female
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Models, Animal
Skin Transplantation*
Transplantation Chimera / immunology*
Transplantation Tolerance / immunology*
Transplantation, Homologous