Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis

Methods Mol Biol. 2018;1773:137-146. doi: 10.1007/978-1-4939-7799-4_11.

Abstract

Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Adipose tissue stromal vascular cells or primary adipocytes derived from murine adipose tissue and grown in culture are essential tools for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells along with protocols for inducing adipogenesis in this cell population or isolating the adipose stromal vascular fraction cells for flow cytometric analysis.

Keywords: Adipogenesis; Adipose-derived stem cells (ASCs); Collagenase; Flow cytometry; Hematopoietic stem cells (HSCs); Isolation; Mesenchymal stem cells (MSCs); Murine; Stromal vascular fraction (SVF).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipocytes / cytology*
  • Adipocytes / drug effects
  • Adipocytes / immunology*
  • Adipogenesis / drug effects
  • Adipogenesis / immunology*
  • Adipose Tissue / cytology
  • Adipose Tissue / drug effects
  • Adipose Tissue / immunology
  • Animals
  • Collagenases / pharmacology
  • Female
  • Flow Cytometry
  • Humans
  • Male
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / immunology*
  • Mice
  • Rosiglitazone / pharmacology
  • Time Factors

Substances

  • Rosiglitazone
  • Collagenases