Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

Methods Mol Biol. 2018;1773:155-165. doi: 10.1007/978-1-4939-7799-4_13.


Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.

Keywords: Adipose-derived stem cells (ASCs); Biopsy; Collagenase; Expansion; Human; Isolation; Lipoaspirate; Mesenchymal stem cells (MSCs); Stromal vascular fraction (SVF).

MeSH terms

  • Adipocytes / cytology*
  • Adipocytes / drug effects
  • Adipose Tissue / cytology*
  • Adipose Tissue / drug effects
  • Azo Compounds / pharmacology
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cell Separation / methods*
  • Collagenases / pharmacology
  • Cryopreservation
  • Humans
  • Lipectomy*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Microscopy, Fluorescence
  • Primary Cell Culture
  • Staining and Labeling


  • Azo Compounds
  • Collagenases
  • collagenase 1
  • oil red O