Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method

Methods Mol Biol. 2018;1773:167-171. doi: 10.1007/978-1-4939-7799-4_14.

Abstract

Adipose tissue provides valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely used adipose-derived stromal/stem cells (ASCs) isolation protocol involves enzymatic digestion with collagenase. However, the yield of the method is poor even impossible to collect enough stromal vascular fraction (SVF) for expansion when the sample size is small, for instance only new born mice is available for cell culture. Here, we describe an efficient protocol for the isolation and expansion of ASCs using explant culture.

Keywords: Adipose-derived stromal/stem cells (ASCs); Collagenase; Explant; Mesenchymal stem cells (MSCs); Murine.

MeSH terms

  • Adipocytes / cytology*
  • Adipose Tissue / cytology*
  • Animals
  • Cell Adhesion
  • Cell Differentiation
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • Mice
  • Microscopy, Phase-Contrast
  • Primary Cell Culture / methods*
  • Rats
  • Tissue Engineering / methods*