Hsp90 Interacts With Tm-22 and Is Essential for Tm-22-Mediated Resistance to Tobacco mosaic virus

Lichao Qian; Jinping Zhao; Yumei Du; Xijuan Zhao; Meng Han; Yule Liu

doi:10.3389/fpls.2018.00411

Hsp90 Interacts With Tm-2² and Is Essential for Tm-2²-Mediated Resistance to Tobacco mosaic virus

Front Plant Sci. 2018 Apr 10:9:411. doi: 10.3389/fpls.2018.00411. eCollection 2018.

Authors

Lichao Qian¹, Jinping Zhao^{1

2}, Yumei Du¹, Xijuan Zhao¹, Meng Han¹, Yule Liu¹

Affiliations

¹ MOE Key Laboratory of Bioinformatics, Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
² Texas A&M AgriLife Research and Extension Center at Dallas, Texas A&M University, Dallas, TX, United States.

Abstract

The tomato resistance gene Tm-2² encodes a coiled coil-nucleotide binding site-leucine rich repeat type resistance protein and confers effective immune response against tobamoviruses by detecting the presence of viral movement proteins (MPs). In this study, we show that the Nicotiana benthamiana Heat shock protein 90-kD (Hsp90) interacts with Tm-2². Silencing of Hsp90 reduced Tm-2²-mediated resistance to Tobacco mosaic virus (TMV) and the steady-state levels of Tm-2² protein. Further, Hsp90 associates with SGT1 in yeast and in plant cells. These results suggest that Hsp90-SGT1 complex takes part in Tm-2²-mediated TMV resistance by functioning as chaperone to regulate Tm-2² stability.

Keywords: Hsp90; NBS-LRRs; Nicotiana benthamiana; SGT1; Tm-22; Tobacco mosaic virus; immunity; plant-virus interaction.