The Effect of the 50 g Glucose Challenge Test on The Thiol/Disulfide Homeostasis in Pregnancy

Fetal Pediatr Pathol. 2018 Jun;37(3):147-156. doi: 10.1080/15513815.2018.1453002. Epub 2018 Apr 25.


Aim: A 50 g glucose challenge test (GCT) is recommended for screening all pregnant women for gestational diabetes mellitus. In this study, the effect of GCT on the thiol/disulfide balance was investigated.

Methods: One-hundred women that underwent a 50 g GCT at 24-28 weeks of gestation (63 positive and 37 negative results) were evaluated in terms of thiol/disulfide in serum samples at test hours 0 and 1.

Results: Compared to the baseline values (hour 0), after the glucose load (hour 1), the thiol and native thiol/total thiol (p < 0.0001) of the GCT-positive women were reduced whereas the values of glucose, disulfide, disulfide/native thiol, disulfide/total thiol (p < 0.0001) and total thiol increased (p = 0.018).

Conclusion: In GCT-positive pregnant individuals, the glucose load increases oxidative stress by changing the thiol/disulfide homeostasis. Such an effect is not observed in healthy pregnancies.

Keywords: 50 g glucose challenge test; Gestational diabetes mellitus; Oral glucose tolerance test; Oxidative stress; Thiol/disulfide homeostasis.

MeSH terms

  • Adult
  • Blood Glucose / analysis
  • Case-Control Studies
  • Diabetes, Gestational / blood*
  • Diabetes, Gestational / diagnosis*
  • Disulfides / blood*
  • Female
  • Glucose Tolerance Test / methods
  • Homeostasis / physiology
  • Humans
  • Oxidative Stress / physiology*
  • Pregnancy
  • Prospective Studies
  • Sulfhydryl Compounds / blood*


  • Blood Glucose
  • Disulfides
  • Sulfhydryl Compounds