Single-Cell RNA-Seq Reveals Transcriptional Heterogeneity in Latent and Reactivated HIV-Infected Cells

Cell Rep. 2018 Apr 24;23(4):942-950. doi: 10.1016/j.celrep.2018.03.102.


Despite effective treatment, HIV can persist in latent reservoirs, which represent a major obstacle toward HIV eradication. Targeting and reactivating latent cells is challenging due to the heterogeneous nature of HIV-infected cells. Here, we used a primary model of HIV latency and single-cell RNA sequencing to characterize transcriptional heterogeneity during HIV latency and reactivation. Our analysis identified transcriptional programs leading to successful reactivation of HIV expression.

Keywords: HIV; cure; heterogeneity; inducible cell; latency; reactivation; scRNA-seq; signature; single cell; transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Female
  • Gene Expression Regulation, Viral / physiology*
  • HIV Infections / genetics
  • HIV Infections / metabolism*
  • HIV-1 / physiology*
  • Humans
  • Male
  • Sequence Analysis, RNA*
  • Virus Activation / physiology*
  • Virus Latency / physiology*