Activated Ductal Proliferation Induced by N-Nitroso bis (2-oxopropyl)amine in Fat-infiltrated Pancreas of KK- A y Mice

In Vivo. May-Jun 2018;32(3):499-505. doi: 10.21873/invivo.11267.

Abstract

Background/aim: Our aim was to investigate whether tissue with fatty infiltration within the lobes of the pancreas (scattered FI) is sensitive to carcinogen-induced pancreatic ductal proliferation.

Materials and methods: Seven-week-old female C57BL/6J, C57BL/6J-Ay, KK-Ay, and ICR mice were subcutaneously treated with N-nitrosobis(2-oxopropyl) amine at a dose of 80 mg/kg body weight, and the differences in damage-induced cell proliferation and their biochemical data were compared 2 days after.

Results: Scattered FI in the pancreas was obvious only in KK-Ay mice, which have high serum lipid, leptin and insulin levels, and cell proliferation both in pancreatic and common bile ducts was enhanced only in KK-Ay mice by the carcinogen treatment.

Conclusion: Scattered FI in the pancreas per se can be an important factor for carcinogenesis. The genetic background causing scattered FI of the pancreas should be further investigated.

Keywords: Fatty infiltration; mouse; pancreatic cancer; proliferation; type 2 diabetes mellitus.

MeSH terms

  • Animals
  • Biomarkers
  • Carcinogens / pharmacology*
  • Cell Proliferation / drug effects
  • Cell Transformation, Neoplastic / chemically induced*
  • Cell Transformation, Neoplastic / pathology*
  • Cricetinae
  • Disease Models, Animal
  • Female
  • Immunohistochemistry
  • Mice
  • Nitrosamines / pharmacology*
  • Pancreas / drug effects*
  • Pancreas / metabolism
  • Pancreas / pathology*
  • Pancreatic Ducts / drug effects
  • Pancreatic Ducts / metabolism
  • Pancreatic Ducts / pathology
  • Pancreatic Neoplasms / etiology
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / pathology

Substances

  • Biomarkers
  • Carcinogens
  • Nitrosamines
  • nitrosobis(2-oxopropyl)amine