Abstract
Four new compounds, phomalichenones A-D (1-4), and seven known compounds (5-11) were isolated from the cultures of an endolichenic fungus Phoma sp. EL002650. Their structures were determined by the analysis of their spectroscopic data (NMR and MS). Compounds 1 and 6 inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. In addition, compound 1 diminished the protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and decreased the mRNA expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin(IL)-1β, and IL-6.
MeSH terms
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Animals
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Anti-Inflammatory Agents / metabolism*
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Anti-Inflammatory Agents / pharmacology*
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Ascomycota / metabolism*
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Cell Line
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Cyclooxygenase 2 / biosynthesis
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Cytokines / genetics
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Interleukin-1beta / genetics
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Interleukin-6 / genetics
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Lipopolysaccharides
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Macrophages / metabolism*
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Mice
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / biosynthesis*
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RAW 264.7 Cells
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RNA, Messenger / biosynthesis
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Tumor Necrosis Factor-alpha / genetics
Substances
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Anti-Inflammatory Agents
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Cytokines
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IL1B protein, mouse
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Interleukin-1beta
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Interleukin-6
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Lipopolysaccharides
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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interleukin-6, mouse
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Nitric Oxide
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2