Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics

Plant Physiol. 2018 Jun;177(2):476-489. doi: 10.1104/pp.18.00293. Epub 2018 Apr 27.


Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis / physiology
  • Chemical Fractionation
  • Chromatography, Liquid
  • Metabolomics / methods*
  • Plant Growth Regulators / analysis*
  • Plant Growth Regulators / isolation & purification
  • Plant Growth Regulators / metabolism
  • Plant Roots / metabolism
  • Plant Shoots / metabolism
  • Reproducibility of Results
  • Salt Stress
  • Tandem Mass Spectrometry / methods*


  • Plant Growth Regulators