Effect of hCG and Ovaprim™ on reproductive characteristics of male Levantine scraper, Capoeta damascina (Valenciennes, 1842)

Theriogenology. 2018 Jul 15;115:45-56. doi: 10.1016/j.theriogenology.2018.04.017. Epub 2018 Apr 20.

Abstract

Species richness and abundance within the genus Capoeta has been depleted. As such, there is great need for developing assisted reproductive technologies for controlling reproduction in captivity. Here, we conducted in vivo studies with single administrations of human chorionic gonadotropin (hCG) and Ovaprim™ [(D-Arg6, Pro9NEt)-sGnRH + domperidone] in wild-caught Levantine scraper, Capoeta damascina and then evaluated milt characteristics, fertilization success, serum sex steroids, and spermatogenesis via histological testicular development. Spermiation responses were significantly stronger for Ovaprim injected fish than those injected with hCG or saline. hCG had a negative effect on milt quality by reducing the percentage of motile sperm and fertilization success at 12-48 h post injection (hpi), which was not observed after treatment with Ovaprim or the saline injection. Hormonal therapy resulted in higher sperm densities and spermatocrit, although sperm longevity was not impacted. Sex steroids were not impacted by hCG or saline injection, but Ovaprim effectively induced androgen and progestin release, as evident by higher serum levels of testosterone, and 17α,20β-dihydroxy-4-pregnen-3-one. Consequently, their levels peaked at 12 hpi, which coincided with maximal milt production. Histological analysis of the testes and quantification of germ cell types revealed that Ovaprim significantly stimulated spermiogenesis, as a higher number of accumulated spermatozoa were observed at 12 h and 24 hpi. Testes from saline and hCG-injected fish remained unchanged through the experiment, and contained all stages of germ cells, predominantly spermatocytes with few spermatozoa. In conclusion, Ovaprim treatment successfully induced steroidogenesis and maturation of spermatogenic germ cells, leading to spermiation and milt production without having any negative impacts on sperm quality and fertility in wild-caught C. damascina.

Keywords: Hormonal treatment; Milt quality; Sex steroids; Testes histology.

MeSH terms

  • Androgens / metabolism
  • Animals
  • Chorionic Gonadotropin / pharmacology*
  • Cyprinidae / physiology*
  • Domperidone / pharmacology*
  • Drug Combinations
  • Fertilization / drug effects
  • Gonadal Steroid Hormones / blood
  • Gonadotropin-Releasing Hormone / pharmacology*
  • Male
  • Progestins / metabolism
  • Reproduction / drug effects*
  • Sperm Count
  • Spermatogenesis / drug effects
  • Spermatozoa / drug effects
  • Spermatozoa / physiology

Substances

  • Androgens
  • Chorionic Gonadotropin
  • Drug Combinations
  • Gonadal Steroid Hormones
  • Progestins
  • ovaprim
  • Gonadotropin-Releasing Hormone
  • Domperidone