Fish Oil Ameliorates High-Fat Diet Induced Male Mouse Reproductive Dysfunction via Modifying the Rhythmic Expression of Testosterone Synthesis Related Genes

Int J Mol Sci. 2018 Apr 29;19(5):1325. doi: 10.3390/ijms19051325.


The present study aims to investigate the protective effects of ω-3 polyunsaturated fatty acids (ω-3PUFAs) against high-fat diet induced male mouse reproductive dysfunction and to explore circadian regulation mechanisms. Male C57BL/6 mice were randomly divided into three groups and fed a normal chow diet (control group, CON), a high-fat diet (HFD group) or a HFD supplemented with fish oil (FO group) for 12 weeks. After 12 weeks of feeding, the body weight and the ratio of perinephric and epididymal fat weight to body weight were significantly higher in the HFD group compared with the CON group. The supplement of fish oil rich in ω-3PUFAs only slightly reduced the HFD-induced obesity but remarkably ameliorated HFD-induced dyslipidemia, sexual hormones disorder, testicle lesions and germ cell apoptosis. Fish oil supplementation restored the expression of steroid synthesis associated genes in HFD fed mouse and flattened the HFD-induced oscillations in circadian genes' expression. Fish oil supplementation prevented HFD-induced male mouse reproductive dysfunction and modified the rhythmic expression of testosterone synthesis related genes.

Keywords: circadian rhythms; fish oil; high-fat diet; reproduction function.

MeSH terms

  • Animals
  • Apoptosis
  • Circadian Rhythm*
  • Diet, High-Fat / adverse effects
  • Dyslipidemias / drug therapy*
  • Dyslipidemias / etiology
  • Fatty Acids, Omega-3 / administration & dosage
  • Fatty Acids, Omega-3 / pharmacology
  • Fatty Acids, Omega-3 / therapeutic use*
  • Infertility, Male / drug therapy*
  • Infertility, Male / etiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Testis / drug effects
  • Testis / metabolism
  • Testosterone / biosynthesis*
  • Testosterone / genetics


  • Fatty Acids, Omega-3
  • Testosterone