Nuclear location of the p55 subunit of the IL-2 receptor following activation of the HT-2 T helper cell line

Ann Inst Pasteur Immunol. 1988 May-Jun;139(3):237-44. doi: 10.1016/0769-2625(88)90137-7.

Abstract

After mitogenic or antigenic stimulation, T cells express interleukin-2 receptors (IL-2R). The mechanism and control of signal transduction following binding of IL-2 to IL-2R are poorly understood. Using two rat monoclonal antibodies (5A2 and 7D4) specific for two distinct epitopes of the p55 subunit of mouse IL-2R, we have studied the cellular localization of this molecule by immunocytochemistry during the IL-2-mediated activation of mouse T helper cell clone HT-2. During the activation cycle, nuclear staining for the p55 subunit of the IL-2 receptor was transiently observed. It is suggested that the transient nuclear location of the IL-2R may play a critical role in the control of T-cell activation, proliferation and/or differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Line
  • Cell Nucleus / immunology
  • Cell Nucleus / metabolism
  • Immunohistochemistry
  • Lymphocyte Activation
  • Mice
  • Receptors, Interleukin-2 / metabolism*
  • T-Lymphocytes, Helper-Inducer / immunology*
  • T-Lymphocytes, Helper-Inducer / metabolism

Substances

  • Antibodies, Monoclonal
  • Receptors, Interleukin-2