Objective: To explore the effects of Kudzu Root plus Cinnamon Granules (KR+C) on prostatic hyperplasia (PH) in mice.
Methods: Sixty 4-week-old Kunming male mice were randomly divided into six groups: blank control, PH model, high-, medium- and low-dose KR+C, and finasteride control. All the mice except those in the blank control group were subcutaneously injected with testosterone propionate (5 mg / ［kg·d］) at 7 days after surgical castration. The animals of different groups were treated intragastrically with different doses of KR+C, finasteride, and normal saline respectively for 3 weeks and then sacrificed for weighing of the prostate, calculation of the prostatic index, observation of the morphological changes in the prostate after HE staining, determination of the expressions of FGF2, Ki67 and TGF-β1 by immunohistochemistry, detection of 5α-reductase activity by ELISA, and measurement of the apoptosis index of the prostatic cells by TUNEL.
Results: Compared with the model controls, the mice of the other groups showed significantly reduced prostatic volume (P <0.05), prostatic index (P <0.05), expressions of FGF2, Ki67 and TGF-β1, and activity of 5 α-reductase (P <0.05), but remarkably increased apoptosis index of the prostatic cells (P <0.05). However, no statistically significant differences were observed in the above parameters between the finasteride control and the three KR+C groups (P>0.05).
Conclusions: KR+C can reduce the prostatic volume of PH mice by decreasing the activity of 5α- reductase, inhibiting the expressions of FGF2, Ki67 and TGF-β1, and promoting the apoptosis of prostatic cells.
目的： 探讨葛根联合肉桂颗粒对小鼠前列腺增生模型的影响。 方法： 将60只4周龄的雄性昆明种小鼠随机分为6组，分为高中低剂量的葛根联合肉桂颗粒组、对照组、造模组、空白组，除空白组外，其余小鼠手术去势7 d后，皮下注射丙酸睾酮5 mg/(kg·d)，同时分别予不同剂量的葛根联合肉桂颗粒、保列治、生理盐水灌胃，持续3周后断颈处死小鼠，摘取前列腺后称重，通过计算前列腺指数（PI），HE染色后观察镜下前列腺形态学变化，免疫组化法检测前列腺FGF2，Ki67，TGF-β1蛋白的表达，ELISA法检测5α还原酶活性，TUNEL法检测凋亡指数，从而初步探讨葛根联合肉桂颗粒对小鼠前列腺增生模型的影响。 结果： 与造模组比较，各给药组小鼠前列腺湿重、指数均明显减小（P<0.05），FGF2，Ki67，TGF-β1蛋白表达、5α还原酶活性显著降低（P<0.05），细胞凋亡指数明显增高（P<0.05）；中高剂量的葛根联合肉桂颗粒组与保列治对照组相比，各项指标无统计学差异（P>0.05）。 结论： 葛根联合肉桂颗粒可能通过降低5α还原酶活性，抑制FGF2，Ki67，TGF-β1蛋白表达，提高细胞凋亡，从而缩小小鼠前列腺的体积。.
Keywords: Kudzu Root Granules; Kunming mice; Cinnamon Granules; finasteride; prostatic hyperplasia.