The Basic Polymerase Chain Reaction (PCR)

Cold Spring Harb Protoc. 2018 May 1;2018(5). doi: 10.1101/pdb.prot095117.

Abstract

This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR).

MeSH terms

  • DNA / genetics
  • Nucleic Acid Denaturation
  • Polymerase Chain Reaction / methods*

Substances

  • DNA