Myc-nick promotes efferocytosis through M2 macrophage polarization during resolution of inflammation

FASEB J. 2018 Oct;32(10):5312-5325. doi: 10.1096/fj.201800223R. Epub 2018 May 2.


A key event required for effective resolution of inflammation is efferocytosis, which is defined as phagocytic removal of apoptotic cells mostly by macrophages acquiring an alternatively activated phenotype (M2). c-Myc has been reported to play a role in alternative activation of human macrophages and is proposed as one of the M2 macrophage markers. We found that M2-like peritoneal macrophages from zymosan A-treated mice exhibited a marked accumulation of Myc-nick, a truncated protein generated by a Calpain-mediated proteolytic cleavage of full-length c-Myc. Further, ectopic expression of Myc-nick in murine bone marrow-derived macrophages promoted the M2 polarization and, consequently, enhanced their efferocytic capability. Notably, Myc-nick-induced efferocytosis was found to be tightly associated with α-tubulin acetylation by K acetyltransferase 2a (Kat2a/Gcn5) activity. These findings suggest Myc-nick as a novel proresolving mediator that has a fundamental function in maintaining homeostasis under inflammatory conditions.-Zhong, X., Lee, H.-N., Kim, S. H., Park, S.-A., Kim, W., Cha, Y.-N., Surh, Y.-J. Myc-nick promotes efferocytosis through M2 macrophage polarization during resolution of inflammation.

Keywords: c-Myc; peritonitis; phagocytosis; tubulin acetylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Antigens, Differentiation / immunology
  • Bone Marrow Cells / immunology*
  • Bone Marrow Cells / pathology
  • Histone Acetyltransferases / immunology
  • Inflammation / immunology
  • Inflammation / pathology
  • Macrophages, Peritoneal / immunology*
  • Macrophages, Peritoneal / pathology
  • Mice
  • Proto-Oncogene Proteins c-myc / immunology*
  • Tubulin / immunology
  • p300-CBP Transcription Factors / immunology


  • Antigens, Differentiation
  • Myc protein, mouse
  • Proto-Oncogene Proteins c-myc
  • Tubulin
  • Histone Acetyltransferases
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor