Proteome-wide mapping of PQS-interacting proteins in Pseudomonas aeruginosa

Chem Sci. 2018 Jan 19;9(8):2290-2294. doi: 10.1039/c7sc04287f. eCollection 2018 Feb 28.

Abstract

The opportunistic human pathogen Pseudomonas aeruginosa secretes 2-heptyl-3-hydroxy-4-quinolone (PQS), a quorum sensing (QS) signal that regulates the expression of numerous virulence genes. Here we report the development and application of chemical probes to globally map quinolone binding proteins. The revealed quinolone interactome contains both known as well as newly identified virulence factors and presents new targets for the treatment of bacterial infections.