Identification of a high-affinity receptor for interleukin-1 beta in rat brain

Biochem Biophys Res Commun. 1988 Oct 14;156(1):61-7. doi: 10.1016/s0006-291x(88)80805-2.

Abstract

A single type of high-affinity binding sites for IL-1 beta was identified in the rat hypothalamus (Kd = 1.0 +/- 0.2 nM) and cerebral cortex (Kd = 1.3 +/- 0.2 nM), but not in the pituitary. The maximum binding capacity (Bmax) in the hypothalamus (Bmax = 75.4 +/- 10.8 fmol/mg protein) was 4 times greater than in the cerebral cortex (Bmax = 17.2 +/- 1.5 fmol/mg protein). Neither various neuropeptides nor IL-2 appeared to influence the binding of [125I]IL-1 beta to the hypothalamic membrane preparations. The potency of unlabeled IL-1 alpha to replace the binding of [125I]IL-1 beta to the hypothalamic membrane preparations was considerably less than that of unlabeled IL-1 beta. These findings indicate that IL-1 beta receptors are heterogeneously distributed in the central nervous system and that IL-1 alpha does not bind with IL-1 beta receptors in the brain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Binding, Competitive
  • Cell Membrane / immunology
  • Cerebral Cortex / immunology*
  • Humans
  • Hypothalamus / immunology*
  • Interleukin-1 / metabolism*
  • Kinetics
  • Male
  • Organ Specificity
  • Pituitary Gland / immunology
  • Rats
  • Rats, Inbred Strains
  • Receptors, Immunologic / isolation & purification
  • Receptors, Immunologic / metabolism*
  • Receptors, Interleukin-1
  • Recombinant Proteins / metabolism*
  • Reference Values

Substances

  • Interleukin-1
  • Receptors, Immunologic
  • Receptors, Interleukin-1
  • Recombinant Proteins