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Case Reports
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Cystic Echinococcosis in a Domestic Cat (Felis Catus) in Italy

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Case Reports

Cystic Echinococcosis in a Domestic Cat (Felis Catus) in Italy

Piero Bonelli et al. Parasite.

Abstract

Echinococcus granulosus sensu lato is a zoonotic agent with a life cycle consisting of definitive hosts (dogs and wild carnivores), and intermediate hosts (usually ungulates). Other animals and humans may accidentally ingest eggs and contract cystic echinococcosis, acting as aberrant hosts. A 3-year-old neutered female cat was brought to a veterinary practice in Sassari (Italy) with abdominal distension. Ultrasound showed multiple intraperitoneal vesicles, which on laparotomy were found to be metacestodes of E. granulosus. Videos of the extraction of cysts are provided. Phylogenetic analysis based on a fragment of the cytochrome oxidase subunit 1 (cox1) mitochondrial gene identified the isolate as E. granulosus sensu stricto genotype G1, the most common genotype circulating in Europe and the Mediterranean basin. This is the first case report of cystic echinococcosis in domestic cats from Italy.

Figures

Figure 1
Figure 1
Abdominal ultrasound images. Hydatids either attached to the omentum (A) or apparently free in the peritoneal cavity (B) with anechoic content and delimited by a hyperechoic rim. Particular of a large peritoneal hydatid with evident bilaminated structure of the wall appearing as a double echogenic line separated by a hypoechogenic space (C).
Figure 1
Figure 1
Abdominal ultrasound images. Hydatids either attached to the omentum (A) or apparently free in the peritoneal cavity (B) with anechoic content and delimited by a hyperechoic rim. Particular of a large peritoneal hydatid with evident bilaminated structure of the wall appearing as a double echogenic line separated by a hypoechogenic space (C).
Figure 2
Figure 2
Gross and histopathological features of hydatid cysts. Free peritoneal hydatids of different dimensions extracted following laparotomy (A). Peritoneal hydatid tested by period-acid-Schiff (PAS) stain (B). Peritoneal hydatid with laminated layer and a brood capsule budding from the germinal layer (C). Protoscoleces in hydatid cavity. Notice the chain of hooks (arrows) (D). Portion of the spleen occupied by multiple cysts (E). Spleen cysts tested by period-acid-Schiff (PAS) stain (F). Spleen cysts displaying laminated, germinal and adventitial layers with inflammatory and fibrotic reaction (G); Greater magnification of the micrograph G showing the epithelioid rim around the cyst wall, giant cells (arrow) (H). Hematoxylin and eosin staining (H&E). Scale bars 100 μm.
Figure 2
Figure 2
Gross and histopathological features of hydatid cysts. Free peritoneal hydatids of different dimensions extracted following laparotomy (A). Peritoneal hydatid tested by period-acid-Schiff (PAS) stain (B). Peritoneal hydatid with laminated layer and a brood capsule budding from the germinal layer (C). Protoscoleces in hydatid cavity. Notice the chain of hooks (arrows) (D). Portion of the spleen occupied by multiple cysts (E). Spleen cysts tested by period-acid-Schiff (PAS) stain (F). Spleen cysts displaying laminated, germinal and adventitial layers with inflammatory and fibrotic reaction (G); Greater magnification of the micrograph G showing the epithelioid rim around the cyst wall, giant cells (arrow) (H). Hematoxylin and eosin staining (H&E). Scale bars 100 μm.
Figure 3
Figure 3
Phylogenetic tree constructed by the Neighbour-Joining method based on partial nucleotide sequences of the cytochrome oxidase subunit 1 (cox1) mitochondrial gene of Echinococcus granulosus sensu lato (s.l.). The phylogenetic analysis includes the sequence recovered from the domestic cat (MG722980) and GenBank sequences of E. granulosus s.l. of different genotypes: E. granulosus sensu stricto (s.s.) genotype 1 (G1) (JF513058), E. granulosus s.s. G2 (JF513059), E. granulosus s.s. G3 (JF513060), E. equinus G4 (AF346403), E. ortleppi G5 (AB235846), E. canadensis G6 (AB208063), E. canadensis G7 (AB235847), E. canadensis G8 (AB235848), E. canadensis G10 (AB745463). A Taenia solium sequence (AY211880) was used as an outgroup.
Figure 3
Figure 3
Phylogenetic tree constructed by the Neighbour-Joining method based on partial nucleotide sequences of the cytochrome oxidase subunit 1 (cox1) mitochondrial gene of Echinococcus granulosus sensu lato (s.l.). The phylogenetic analysis includes the sequence recovered from the domestic cat (MG722980) and GenBank sequences of E. granulosus s.l. of different genotypes: E. granulosus sensu stricto (s.s.) genotype 1 (G1) (JF513058), E. granulosus s.s. G2 (JF513059), E. granulosus s.s. G3 (JF513060), E. equinus G4 (AF346403), E. ortleppi G5 (AB235846), E. canadensis G6 (AB208063), E. canadensis G7 (AB235847), E. canadensis G8 (AB235848), E. canadensis G10 (AB745463). A Taenia solium sequence (AY211880) was used as an outgroup.

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References

    1. Alvarez Rojas CA, Romig T, Lightowlers MW. 2014. Echinococcus granulosus sensu lato genotypes infecting humans – review of current knowledge of current knowledge. International Journal for Parasitology, 44, 9-18. - PubMed
    1. Armua-Fernandez MT, Castro OF, Crampet A, Bartzabal A, Hofmann-Lehmann R, Grimm F, Deplazes P. 2014. First case of peritoneal cystic echinococcosis in a domestic cat caused by Echinococcus granulosus sensu stricto (genotype 1) associated to feline immunodeficiency virus infection. Parasitology International, 63, 300-302. - PubMed
    1. Bowels J, McManus D. 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular & Biochemical Parasitology, 54, 165-174. - PubMed
    1. Brundu D, Piseddu T, Stegel G, Masu G, Ledda S, Masala G. 2014. Retrospective study of human cystic echinococcosis in Italy based on the analysis of hospital discharge records between 2001 and 2012. Acta Tropica, 140, 91-96. - PubMed
    1. Burgu A, Vural SA, Sarimehmetoglu O. 2004. Cystic echinococcosis in a stray cat. Veterinary Records, 155, 711-712. - PubMed

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