STAT1-mediated Upregulation of lncRNA LINC00174 Functions a ceRNA for miR-1910-3p to Facilitate Colorectal Carcinoma Progression Through Regulation of TAZ

Gene. 2018 Aug 5;666:64-71. doi: 10.1016/j.gene.2018.05.001. Epub 2018 May 3.

Abstract

Several of the thousands of human long noncoding RNAs (lncRNAs) have been functionally characterized, yet their potential involvement in colorectal carcinoma (CRC) remains poorly understood. In present study, we aim to investigate the role of lncRNA LINC00174 in CRC carcinogenesis. We observed that increased expression of LINC00174 in CRC tissues and cells in comparison to their corresponding controls. Moreover, the aberrant overexpression of LINC00174 indicated the poor prognosis of CRC patients. Silence of LINC00174 was able to repress CRC cell growth in vitro and in vivo. We first reported that transcription factor STAT1 mediated LINC00174 expression in CRC. In addition, rescue assay was performed to further confirm that LINC00174 contributed to CRC progression by regulating miR-1910-3p/TAZ signal pathway. Taken together, our study discovered the oncogenic role of LINC00174 in clinical specimens and cellular experiments, showing the potential LINC00174/miR-1910-3p/TAZ pathway. This results and findings provide a novel insight for CRC tumorigenesis.

Keywords: Colorectal carcinoma; LINC00174; TAZ; Tumorigenesis; miR-1910-3p.

MeSH terms

  • Cell Line, Tumor
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / mortality
  • Colorectal Neoplasms / pathology
  • CpG Islands
  • DNA Methylation
  • Disease Progression
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Kaplan-Meier Estimate
  • MicroRNAs / physiology*
  • Prognosis
  • RNA Interference
  • RNA, Long Noncoding / physiology*
  • STAT1 Transcription Factor / physiology*
  • Transcription Factors
  • Up-Regulation

Substances

  • Intracellular Signaling Peptides and Proteins
  • MIRN1910-3p microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • STAT1 Transcription Factor
  • STAT1 protein, human
  • Transcription Factors
  • WWTR1 protein, human